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For intrinsic spontaneous action potential firing recordings, a potassium-based internal solution was used (in mM: 128 K-gluconate, 10 HEPES, 10 sodium phosphocreatine, 4 MgCl2, 4 sodium ATP, 0.4 sodium GTP, 3 ascorbic acid, 1 EGTA, 4 mg/mL biocytin)

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The National Institute on Aging has published extensively on the importance of strength training for adults, particularly for preserving muscle mass during periods of weight change