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Neuron 2019;102:653-67.ArticlePubMedPMC 93

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LDL-cholesterol uptake by HepG2 hepatic cells Ability of anti-PCSK9 antibodies and peptide-antibody fusions to block PCSK9 activity and restore LDL-C uptake was tested in vitro by plating in 96-well plates 2 × 10 4 HepG2 cells per well in DMEM medium supplemented with 10% lipoprotein deficient serum and cultured overnight