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To obtain the GLP-1R–G s complex with good stability and homogeneity, the NanoBiT tethering strategy was used 15 , in which the C terminus of GLP-1R was directly attached to the LgBiT subunit 16 (Promega), followed by a tobacco etch virus (TEV) protease cleavage site and a double maltose binding protein (MBP) tag (Fig
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