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⚠️ Common Reasons for SST Failure ❌ Incorrect mobile phase preparation ❌ Air bubbles in the pump or detector flow cell ❌ Deteriorated or contaminated HPLC column ❌ Incorrect flow rate, wavelength, or column temperature ❌ Standard solution degradation or preparation error 💡 Pro Tip Always inject the system suitability standard 5–6 times (or as specified in the method) and verify that all chromatographic parameters meet the predefined acceptance criteria before injecting any samples

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If outside these windows, wait until the next regularly scheduled dose

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