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INS-1 832/3 cells (a gift from Prof Christopher Newgard, Duke University) (Hohmeier et al ., 2000) were maintained in RPMI at 11 mM glucose, supplemented with 10% FBS, 10 mM HEPES, 1 mM pyruvate, 1 mM pyruvate, 50 μM β-mercaptoethanol and 1% penicillin/streptomycin
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However low, a certain degree of excretion in human milk is expected as animal data is a reliable predictor of the presence of a drug and/or its active metabolite(s) in human milk ( Supplement 3 ) but the animal data suggest a systemic effect
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